Coding

Part:BBa_K4578001:Design

Designed by: Kelly Yoon   Group: iGEM23_USAFA   (2023-10-05)


OspA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
    Illegal BglII site found at 693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 81
    Illegal EcoRI site found at 642
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used with INP to produce expression on surface of E. coli


Source

"Temporal Changes in Outer Surface Proteins A and C of the Lyme Disease-Associated Spirochete, Borrelia burgdorferi, during the Chain of Infection in Ticks and Mice" DOI: 10.1128/jcm.38.1.382-388.2000

References